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ktrB [2019-05-02 17:56:46]
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ktrB [2019-05-02 17:56:46]

high affinity potassium channel [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], integral membrane subunit
locus
BSU31100
pI
10.12
mw
48.27 kDa
protein length
445 aa Sequence Blast
gene length
1338 bp Sequence Blast
function
potassium uptake
product
high affinity potassium channel [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], integral membrane subunit
essential
no
synonyms
yubG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,189,089 3,190,426

    Phenotypes of a mutant

  • a [gene|search|kimA ][gene|search|ktrA ][gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of potassium

    • Paralogous protein(s)

  • [protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD]

    • Kinetic information

  • the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] channel has a high affinity for potassium,this is determined by [protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] [pubmed|30753894]

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex) [Pubmed|23598340]

    • [SW|Localization]

  • integral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]
    description
    [Pubmed|12562800]

    regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP, in [regulon|ydaO riboswitch|ydaO riboswitch]
    • view in new tab

    additional information

  • growth at extreme potassium limitation results in the acquisition of promoter mutations with increased [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] expression [pubmed|28679749]

    • Biological materials

    Mutant

  • MGNA-A227 (yubG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/227 NBRP B. subtilis, Japan]
  • 1A954 ( ''ktrB''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
  • GHB1 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Erhard Bremer]'s lab
  • GP92 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2083 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3'' [gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2498 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''spc'' [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]::''cat''), available in [SW|Jörg Stülke]'s lab
  • BKE31100 ([gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTGTCTACATCCCTT, downstream forward: _UP4_TGATATCAAAAAAATCCGGC
  • BKK31100 ([gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTGTCTACATCCCTT, downstream forward: _UP4_TGATATCAAAAAAATCCGGC
  • GP2716 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''spc''), available in [SW|Jörg Stülke]'s lab
  • GP3064 ([gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''kan''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2992: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP2277 (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28679749]

    • labs

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 25869574,21680052,27935846,25838295,28825218

    • Original publications

  • 12562800,20511502,17932047,23086297,23598340,16990138,24141192,26771197,28420751,28504641,28679749,30753894